By Revilo P. Oliver
From a speech by means of Dr. Revilo P. Oliver,
University of Illinois Professor of the Classics,
in March, 1959, earlier than the
DAUGHTERS OF the yank REVOLUTION
Annual Illinois kingdom Convention
Supplementary notes are supplied to document
and, in a couple of circumstances, to extend and explain
statements made within the speech on March 12, 1959.
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Additional resources for All America Must Know the Terror that Is Upon Us
2) Place tissue in fixative immediately after removal from the animal. 5 cm. Larger pieces can be cut up in the fixative using sharp razors or scalpels. , will harden after 15-30min in fixative and can then be cut down to the correct size. (4) Use a volume of fixative at least 10-15 times the volume of the tissue. (5) Use freshly made up fixatives. (6) Make sure all fixative is thoroughly washed out before subsequent stages. (e) cation methods exist, but usually acid or chelating agents are used after fixation, for example as folIows: Kristensen fluid Notes Good general fixative especially for cytoplasm; fix for 3-24h and wash overnight in running tap water; then, to remove excess mercuric chloride from tissues, place for 5-6 h in 70% alcohol made dark brown by addition of saturated solution of iodine in 70% alcohol.
Never draw / on both sides of the paper. (6) Do not waste time drawing too many cells oftbe same type. If a layer is 20 cells tbick, draw only a few cells and indicate tbe remainder by a dotted line. n = 60 ,ni" wld. (c) ~ 100ILm x y /\ I I 1111111 10 20 30 40 50 V 10 ILm subdivisions (b) eyepiece graticule with arbitrary linear scale Fig. 2. Measurement of object size. (a) View of specimen through eyepiece graticule. e. one without graticule). (c) Graticule superimposed upon micrometer sUde (after removal ofspecimen) 36 Practical Illustrated Histology (7) Draw only what can be seen under the microscope.
B. Many of the chemicals used in tissue processing are toxic Since most mammalian organs and tissues cannot be examined in situ and are too thick to allow sufficient light to pass through them for microscopic examination, they must first be removed from the animal, cut into thin sections and then stained before their detailed structure can be examined under the compound light microscope. The following few pages contain a very brief outline of tissue preparation for the microscope and include some useful formulae and schedules which will hopefully assist the student in his laboratory work.
All America Must Know the Terror that Is Upon Us by Revilo P. Oliver